This Enzymology Internship is a rigorous, biochemically-focused program designed to provide students with deep technical expertise in the isolation, characterization, and industrial application of biological catalysts. Participants will master the fundamental principles of enzyme kinetics, gaining hands-on experience in determining Michaelis-Menten constants ($K_m$ and $V_{max}$) and understanding the mechanisms of enzyme inhibition and activation. The curriculum covers a broad range of advanced laboratory techniques, including protein extraction, various chromatography methods for enzyme purification, and spectrophotometric assays to measure catalytic activity under varying $pH$ and temperature conditions. By exploring modern applications such as enzyme immobilization, site-directed mutagenesis for protein engineering, and the use of biocatalysts in green chemistry, the program prepares interns for high-level roles in pharmaceutical drug design, clinical diagnostics, and sustainable industrial manufacturing.